Seahorse XF Real-Time ATP Rate Assay Kit

The Agilent Seahorse ATP Real-Time rate assay is the only assay that quantifies the rate of adenosine triphosphate (ATP) production from glycolysis and mitochondria simultaneously using label-free technology in live cells. The real-time kinetic quantification delivers a dynamic picture of cellular bioenergetics, providing researchers with unique insights into cellular phenotype and function. This assay should be performed using XF pH-defined media. Please refer to the assay user guide to select the correct media.

Each assay kit contains sufficient materials for six full-plate tests and is available in two packaging sizes. The XF kit is for use with Agilent Seahorse XF Pro, XFe96, and XFe24 Analyzers. The XFp kit is for use with the Agilent Seahorse XF HS Mini and XFp Analyzers.

XF Assay Kits, Reagents & Cell Assay Media

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Product Details

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Simultaneously measure basal ATP production rates from mitochondrial respiration and glycolysis. Total ATP production rates can also be obtained
Assess the effect of compounds on ATP production rates
Generate real-time kinetic data in live cells, allowing users to assess the interplay and relationship between energy metabolism and cellular functions
Use with Agilent Seahorse XF DMEM medium, pH 7.4 (Cat. No. 103575-100) or Seahorse XF RPMI medium, pH 7.4 (Cat. No. 103576-100)
Reconstitute kit components directly in the assay medium, removing the need for DMSO or other solvents
Eliminate the cost associated with failed experiments since each lot is rigorously tested and validated with functional assays to deliver consistent robust results
Upload your assay result file to Agilent Seahorse Analytics to calculate and report parameters of the XF Real-Time ATP Rate assay, share with collaborators, or export data

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Technology

Automatic measurement of energy metabolism in real time

Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.

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Rapid Bioenergetic Functional Screening of Anticancer Drug Candidates

This application note introduces a workflow that uses the XF Real-Time ATP rate assay data for identifying metabolic modulator and drug targets.

Application Notes
English
30 Jan 2023
1.58 MB
PDF

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Seahorse XFp Real-Time ATP Rate Assay Kit

Material Safety Data Sheet

Material Safety Data Sheets
English
03 Jul 2024
142.73 KB
PDF

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Agilent Seahorse XF Real-Time ATP Rate Assay Kit, Part Number 103592-100

Material Safety Data Sheet

Material Safety Data Sheets
English
22 Apr 2022
148.90 KB
PDF

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Agilent Seahorse XF Instruments Selection Guide

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Agilent Cell Analysis Publication Database

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Seahorse XF Assay Learning Center

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Customer Reviews for Seahorse XF Real-Time ATP Rate Assay Kit

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The Agilent Seahorse XF Pro platform

Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.

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